|
Status |
Public on Feb 04, 2024 |
Title |
Control plants [SAMPLE 1-1] |
Sample type |
RNA |
|
|
Source name |
Shoots
|
Organism |
Solanum lycopersicum |
Characteristics |
cell type: Shoots activation agent: Control plants time: 2h
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with AccuPrep® Universal RNA Extraction Kit followed by DNase I treatment.
|
Label |
SYBR Green
|
Label protocol |
Reactions (10 µL final volume) were prepared using 5 µL of LaboPass™ SYBR Green Q Master Kit (Cosmogenetech, Dajeon, Korea). 0.5 pmol of a primer pair, and 0.5 µL of cDNA template
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
Control
|
Data processing |
Ubiquitin was used as the reference. gene expression analysis was performed with the 2^−ΔΔCt method using Bio-Rad CFX Maestro software v.4.0 (Bio-Rad). Baseline and threshold levels were set according to the manufacturer’s instructions.
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|
|
Submission date |
Nov 17, 2023 |
Last update date |
Feb 04, 2024 |
Contact name |
Sujeevan Rajendran |
E-mail(s) |
sujee119@gmail.com
|
Phone |
+821068069010
|
Organization name |
Wonkwang University
|
Street address |
Muwang-ro
|
City |
Iksan |
ZIP/Postal code |
54538 |
Country |
Sri Lanka |
|
|
Platform ID |
GPL33939 |
Series (1) |
GSE248090 |
Real-time quantitative PCR analysis of Tomato stress response |
|