|
| Status |
Public on Feb 04, 2024 |
| Title |
Treated with acid (pH 4.2 water) [SAMPLE 6-2] |
| Sample type |
RNA |
| |
|
| Source name |
roots
|
| Organism |
Solanum lycopersicum |
| Characteristics |
cell type: roots
activation agent: Salt stressed plants
time: 24h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with AccuPrep® Universal RNA Extraction Kit followed by DNase I treatment.
|
| Label |
SYBR Green
|
| Label protocol |
Reactions (10 µL final volume) were prepared using 5 µL of LaboPass™ SYBR Green Q Master Kit (Cosmogenetech, Dajeon, Korea). 0.5 pmol of a primer pair, and 0.5 µL of cDNA template
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
Ubiquitin was used as the reference. gene expression analysis was performed with the 2^−ΔΔCt method using Bio-Rad CFX Maestro software v.4.0 (Bio-Rad). Baseline and threshold levels were set according to the manufacturer’s instructions.
|
| |
|
| Submission date |
Nov 17, 2023 |
| Last update date |
Feb 04, 2024 |
| Contact name |
Sujeevan Rajendran |
| E-mail(s) |
sujee119@gmail.com
|
| Phone |
+821068069010
|
| Organization name |
Wonkwang University
|
| Street address |
Muwang-ro
|
| City |
Iksan |
| ZIP/Postal code |
54538 |
| Country |
Sri Lanka |
| |
|
| Platform ID |
GPL33939 |
| Series (1) |
| GSE248090 |
Real-time quantitative PCR analysis of Tomato stress response |
|
