|
| Status |
Public on Dec 01, 2012 |
| Title |
vastus 4wk FRG1 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis
|
| Organism |
Mus musculus |
| Characteristics |
muscle: vastus lateralis
time point: 4wk
genotype/variation: FRG1 over-expressing
background mouse strain: C57Bl/6J
|
| Growth protocol |
Vastus lateralis and biceps brachii dissected from 4wk and 13wk wild type and FRG1 mice into RNAlater (Ambion).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Rneasy Fibrous Tissue Mini kit (Qiagen), and treated with Dnase I.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Target Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Using Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
probe group file: GPL13185_mjay.pgf
meta-probeset file: GPL13185_mjay.clf
|
| |
|
| Submission date |
Sep 12, 2011 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Lily Shiue |
| E-mail(s) |
lshiue@ucsc.edu
|
| Phone |
831-459-2612
|
| Organization name |
UCSC
|
| Department |
MCD Biology
|
| Lab |
Ares
|
| Street address |
1156 High Street
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platform ID |
GPL13185 |
| Series (1) |
| GSE32073 |
Rbfox1 downregulation and altered Calpain 3 splicing by FRG1 in a mouse model of facioscapulohumeral muscular dystrophy (FSHD). |
|
