|
| Status |
Public on Jan 17, 2024 |
| Title |
ADV 1 |
| Sample type |
protein |
| |
|
| Source name |
HeG2 xenografted
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: HeG2 xenografted in subcutaneus of NOD-SCID mice
treatment: ADV treated
|
| Treatment protocol |
Six-week-old male mice were used for study. Dimethyl sulfoxide (DMSO), ETV, ADV, and TDF were administered intraperitoneally to the mice at a concentration of 90 mg/kg every 3 days. At 7 days after first treatment with the NAs, the mice were injected subcutaneously with 5.0 × 106 HepG2 cells in the right flank. At day 50, tumor volume was evaluated and phosphorylation activity was measured.
|
| Growth protocol |
NOD-SCID mice were purchased from The Jackson Laboratory. Six-week-old male mice were used for study. Dimethyl sulfoxide (DMSO), ETV, ADV, and TDF were administered intraperitoneally to the mice at a concentration of 90 mg/kg every 3 days. At 7 days after first treatment with the NAs, the mice were injected subcutaneously with 5.0 × 106 HepG2 cells in the right flank. At day 50, tumor volume was evaluated and phosphorylation activity was measured.
|
| Extracted molecule |
protein |
| Extraction protocol |
Cell lysates were extracted from each tumor and100 μg total protein was used for labbelling.
|
| Label |
Alexa Fluor 647
|
| Label protocol |
Cell lysates of 100 μg total protein were applied to the array with additional ATP at 30°C for 3 h. After termination of the kinase reaction, the array was washed with Tris-buffered saline containing 0.05% Tween 20 and stained with a 4G10 phosphorylated protein-specific antibody (Merck, #05-1050) and a secondary fluorescein-conjugated antibody (Thermo, #A21235) to detect the phosphorylated tyrosine residues.
|
| |
|
| Hybridization protocol |
Cell lysates of 100 μg total protein were applied to the array with additional ATP at 30°C for 3 h.
|
| Scan protocol |
The array was washed with Tris Buffered Saline with 0.05% Tween20 (TBST) and stained with the 4G10 phosphorylated protein-specific antibody (Merck, #05-1050) and a secondary fluorescein-conjugated antibody (Thermo, #A21235) to detect the phosphorylated tyrosine residues.
|
| Data processing |
Signal data were averaged (n=6), filtered and normalized by BRB-ArrayTools (https://brb.nci.nih.gov/BRB-ArrayTools).
|
| |
|
| Submission date |
Dec 11, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Masao Honda |
| E-mail(s) |
mhondag@gmail.com
|
| Phone |
0762652243
|
| Organization name |
Kanazawa University
|
| Department |
Gastroenterology
|
| Street address |
Takara-Machi 13-1
|
| City |
Kanazawa |
| ZIP/Postal code |
920-8641 |
| Country |
Japan |
| |
|
| Platform ID |
GPL34000 |
| Series (1) |
| GSE249861 |
Nucleos(t)ide analogs for hepatitis B virus infection differentially regulate the growth factor signaling in hepatocytes |
|
