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Sample GSM7966050 Query DataSets for GSM7966050
Status Public on Jan 17, 2024
Title TDF 2
Sample type protein
 
Source name HeG2 xenografted
Organism Homo sapiens
Characteristics tissue: HeG2 xenografted in subcutaneus of NOD-SCID mice
treatment: TDF treated
Treatment protocol Six-week-old male mice were used for study. Dimethyl sulfoxide (DMSO), ETV, ADV, and TDF were administered intraperitoneally to the mice at a concentration of 90 mg/kg every 3 days. At 7 days after first treatment with the NAs, the mice were injected subcutaneously with 5.0 × 106 HepG2 cells in the right flank. At day 50, tumor volume was evaluated and phosphorylation activity was measured.
Growth protocol NOD-SCID mice were purchased from The Jackson Laboratory. Six-week-old male mice were used for study. Dimethyl sulfoxide (DMSO), ETV, ADV, and TDF were administered intraperitoneally to the mice at a concentration of 90 mg/kg every 3 days. At 7 days after first treatment with the NAs, the mice were injected subcutaneously with 5.0 × 106 HepG2 cells in the right flank. At day 50, tumor volume was evaluated and phosphorylation activity was measured.
Extracted molecule protein
Extraction protocol Cell lysates were extracted from each tumor and100 μg total protein was used for labbelling.
Label Alexa Fluor 647
Label protocol Cell lysates of 100 μg total protein were applied to the array with additional ATP at 30°C for 3 h. After termination of the kinase reaction, the array was washed with Tris-buffered saline containing 0.05% Tween 20 and stained with a 4G10 phosphorylated protein-specific antibody (Merck, #05-1050) and a secondary fluorescein-conjugated antibody (Thermo, #A21235) to detect the phosphorylated tyrosine residues.
 
Hybridization protocol Cell lysates of 100 μg total protein were applied to the array with additional ATP at 30°C for 3 h.
Scan protocol The array was washed with Tris Buffered Saline with 0.05% Tween20 (TBST) and stained with the 4G10 phosphorylated protein-specific antibody (Merck, #05-1050) and a secondary fluorescein-conjugated antibody (Thermo, #A21235) to detect the phosphorylated tyrosine residues.
Data processing Signal data were averaged (n=6), filtered and normalized by BRB-ArrayTools (https://brb.nci.nih.gov/BRB-ArrayTools).
 
Submission date Dec 11, 2023
Last update date Jan 17, 2024
Contact name Masao Honda
E-mail(s) mhondag@gmail.com
Phone 0762652243
Organization name Kanazawa University
Department Gastroenterology
Street address Takara-Machi 13-1
City Kanazawa
ZIP/Postal code 920-8641
Country Japan
 
Platform ID GPL34000
Series (1)
GSE249861 Nucleos(t)ide analogs for hepatitis B virus infection differentially regulate the growth factor signaling in hepatocytes

Data table header descriptions
ID_REF
VALUE The values mean the intensities of phosphorylated tyrosine residues. Processed data were log2 transformed.

Data table
ID_REF VALUE
1
2 4.322297573
3 4.165258884
4
5 5.837442875
6
7 4.389156342
9 4.645153046
10 3.86990118
11
12 0.765727699
13 4.407193184
14 6.425750732
15
16 5.572710991
17 5.744979382
18 3.169181108
19
20 4.707720757
21 2.808597565

Total number of rows: 1180

Table truncated, full table size 14 Kbytes.




Supplementary data files not provided

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