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Sample GSM7966146 Query DataSets for GSM7966146
Status Public on Dec 16, 2023
Title Skin normal 91
Sample type genomic
 
Source name Saliva
Organism Homo sapiens
Characteristics gender: F
Extracted molecule genomic DNA
Extraction protocol Extraction of genomic DNA from saliva was performed using MagMAX DNA Multi-Sample Ultra 2.0 Kit on KingFisher™ Flex Purification System.
Label Cy3, Cy5
Label protocol 250ng of genomic DNA was whole-genome amplified in an overnight reaction at 37˚C using amplification master mix (MA1, MA2 and MSM). After incubation the amplified DNA was fragmented with fragmentation mix (FMS), precipitated with isopropanol and precipitation mix (PM1) and resuspended in hybridization buffer (RA1).
 
Hybridization protocol RA1 resuspended DNA was loaded onto Infinium Asian Screening Array-24 v1.0 BeadChip . After incubation 16 - 24 hours at 48˚C, single-base extension and allele-specific staining was performed on a Te-flow chamber rack system (Tecan, Maennedorf, Switzerland).
Scan protocol After allele-specific staining the Beadchip was coated with XC4, dried for 1 hour and scanned by IScan Software (Illumina).
Description Genomic DNA extracted from saliva was genotyped usingInfinium Asian Screening Array-24 v1.0 BeadChip).
gst-skin-91
Data processing Genomic DNA extracted from saliva was genotyped using ASA-24 v1.0 Beadchip (Illumina).
 
Submission date Dec 11, 2023
Last update date Dec 16, 2023
Contact name Tham H Hoang
E-mail(s) tham.hoang@uconn.edu
Organization name Vingroup Big Data Institute
Street address Floor 9th, 458 Minh Khai
City Hanoi
ZIP/Postal code 000000
Country Viet Nam
 
Platform ID GPL33106
Series (1)
GSE248483 A study of genetic variants associated with skin traits in the Vietnamese population

Supplementary file Size Download File type/resource
GSM7966146_205685220120_R10C01_Grn.idat.gz 4.7 Mb (ftp)(http) IDAT
GSM7966146_205685220120_R10C01_Red.idat.gz 4.8 Mb (ftp)(http) IDAT

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