|
| Status |
Public on Apr 01, 2024 |
| Title |
IDH-WT_ATRA_3 |
| Sample type |
SRA |
| |
|
| Source name |
Astrocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Astrocytes
genotype: IDH-WT
treatment: ATRA
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Illumina’s TruSeq RNA Access Library Preparation protocol following the manufacturer’s instruction
Pooled libraries were then sequenced on Illumina NextSeq 500 platform with single-end 76 bp reads, to a target read count of 40 million reads per sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Sample3
|
| Data processing |
Reads were aligned to the human genome (GRCh38) using STAR aligner(v2.7.3a) with default parameters. Gene-count matrices were produced by featureCounts (v2.0.1).
Assembly: GRCh38
Supplementary files format and content: Sample by gene matrix of read counts
|
| |
|
| Submission date |
Jan 04, 2024 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Nduka M Amankulor |
| E-mail(s) |
nduka.amankulor@pennmedicine.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Department of Neurosurgery
|
| Lab |
Amankulor Lab
|
| Street address |
3400 Civic Center Boulevard, 15th Floor
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE252527 |
All-Trans-Retinoic Acid Treatment of IDH Mutant Astrocytes |
|
| Relations |
| BioSample |
SAMN39262865 |
| SRA |
SRX23102194 |
