|
| Status |
Public on Jan 01, 2012 |
| Title |
ECs from EC/NSPC co-culture incubated with DMSO (control) 1 |
| Sample type |
other |
| |
|
| Source name |
Mouse primary brain endothelial cells (Celprogen) grown in co-culture with neural stem/progenitor cells isolated from E14 mouse embryonic brain
|
| Organism |
Mus musculus |
| Characteristics |
cell type: primary brain endothelial cells (Celprogen, San Diego, CA, catalog # 11053-07)
sample type: ECs isolated from the mouse forebrain microvessels and NSPCs from telencephalon of E-14 mouse embryo
treatment: EC/NSPC co-culture treated with DMSO (control)
|
| Treatment protocol |
co-cultures were treated with : PD 173074 (1mM in DMSO), SB431542 (10μM in DMSO), or 2μl/mL DMSO (control), for 24 hours.
|
| Growth protocol |
ECs were grown in transwell co-culture with NSPCs,in growth factor-free NSPC medium with addition of 5% FBS, at 37C, in CO2 incubator
|
| Extracted molecule |
other |
| Extraction protocol |
After 24 hours in transwell co-culture, the ECs and NSPCs were separated and miRNA s were isolated using mirVana miRNA (AB/Ambion) isolation kit
|
| Label |
biotin
|
| Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Following labeling, 21.5 ul of biotin-labeled RNA was hybridized for 16 hr at 48C on Affymetrix miRNA 2.0 Arrays. The miRNA 2.0 Arrays were washed and stained in the Affymetrix Fluidics Station 450 using fludics script FS450_0003.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
miRNA expression data from EC/NSPC co-culture treated with DMSO (control)
|
| Data processing |
Data files were analyzed with Affymetrix miRNAQCtool using RMA background correction and quantile normalization (Default settings)
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Tamara Roitbak |
| E-mail(s) |
troitbak@salud.unm.edu, ggpickett@salud.unm.edu
|
| Phone |
505 272-3401
|
| Fax |
505 272-6091
|
| Organization name |
UNM
|
| Department |
Neurosurgery
|
| Street address |
2212 Lomas NE
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE32352 |
Role of microRNAs in Neural Stem Cell-supported Endothelial Morphogenesis |
|
