|
| Status |
Public on Apr 26, 2024 |
| Title |
CD34+ C3 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow from healthy donors
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34 cells
condition: AML condition, with hMSCs-AML and CD34+ cells from donors
|
| Treatment protocol |
The CD34+ cells were obtained from two conditions of co-culture assays: control condition, with hMSCs and CD34+ cells from donors; and AML condition, with hMSCs-AML and CD34+ cells from donors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from all CD34+ cells used in this study was obtained using the RNeasy Mini Kit (Qiagen, CA, USA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix, USA) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix, USA).
|
| |
|
| Hybridization protocol |
The biotinylated cRNA was subsequently hybridized to a Clariom S Array human (Thermo Scientific, USA).
|
| Scan protocol |
The GeneChip arrays were washed and stained according to the manufacturer’s protocols and scanned using a GeneChip® Scanner 3000 (Affymetrix, USA)
|
| Description |
RMA expression value derived from TAC software
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) software (Affymetrix, USA).
|
| |
|
| Submission date |
Jan 18, 2024 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Renata Binato |
| E-mail(s) |
renata.binato@inca.gov.br
|
| Organization name |
INCA
|
| Street address |
Praça da Cruz Vermelha 23
|
| City |
Rio de Janeiro |
| ZIP/Postal code |
20230130 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE253556 |
Expression profile of bone marrow HSCs from healthy donors |
|
