|
Status |
Public on Oct 10, 2011 |
Title |
#015 |
Sample type |
RNA |
|
|
Source name |
liver
|
Organism |
Homo sapiens |
Characteristics |
ethnic background: Caucasian origin gender: male age: 70 presurgical medication: no smoking: non-smoker alcohol intake: no
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction + RNeasy clean-up with DNase treatment (Qiagen)
|
Label |
biotin
|
Label protocol |
Standard Illumina protocol with biotin and streptavidin-Cy3 detection
|
|
|
Hybridization protocol |
We load cRNA on Illumina Hyb chambersand hybridize for 14 h at 58°C
|
Scan protocol |
We use a Beadarray Reader 500 and scan with Direct Hyb settings
|
Description |
1888857054_C
|
Data processing |
Quantile Normalisation without background correction with Illumina Genomestudio
|
|
|
Submission date |
Sep 30, 2011 |
Last update date |
Nov 03, 2011 |
Contact name |
Michael Bonin |
Organization name |
University Tuebingen
|
Department |
Medical Genetics
|
Lab |
Microarray Facility
|
Street address |
CAlwerstr. 7
|
City |
Tuebingen |
State/province |
BW |
ZIP/Postal code |
72076 |
Country |
Germany |
|
|
Platform ID |
GPL13376 |
Series (1) |
GSE32504 |
Identification of expression quantitative trait loci (eQTL) in human liver |
|