|
| Status |
Public on Oct 10, 2011 |
| Title |
#099 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Homo sapiens |
| Characteristics |
ethnic background: Caucasian origin
gender: male
age: 37
presurgical medication: no
smoking: non-smoker
alcohol intake: no
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction + RNeasy clean-up with DNase treatment (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol with biotin and streptavidin-Cy3 detection
|
| |
|
| Hybridization protocol |
We load cRNA on Illumina Hyb chambersand hybridize for 14 h at 58°C
|
| Scan protocol |
We use a Beadarray Reader 500 and scan with Direct Hyb settings
|
| Description |
1951406052_C
|
| Data processing |
Quantile Normalisation without background correction with Illumina Genomestudio
|
| |
|
| Submission date |
Sep 30, 2011 |
| Last update date |
Nov 03, 2011 |
| Contact name |
Michael Bonin |
| Organization name |
University Tuebingen
|
| Department |
Medical Genetics
|
| Lab |
Microarray Facility
|
| Street address |
CAlwerstr. 7
|
| City |
Tuebingen |
| State/province |
BW |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13376 |
| Series (1) |
| GSE32504 |
Identification of expression quantitative trait loci (eQTL) in human liver |
|
