|
Status |
Public on Apr 19, 2024 |
Title |
T24-hNectin4 (ADC-treated) mouse 2 |
Sample type |
SRA |
|
|
Source name |
xenograft
|
Organisms |
Homo sapiens; Mus musculus |
Characteristics |
tissue: xenograft cell line: T24-hNectin4 treatment: ASG-22C3E at 3.0 mg/kg
|
Treatment protocol |
On Day 15 post implantation, tumor volumes reached ~200 mm3 (calculated using 0.5 × [length] × [width]2), and the mice were treated with a single intraperitoneal (IP) dose of 3 mg/kg enfortumab vedotin. As a control, mice were treated with 3 mg/kg IgG-MMAE.
|
Growth protocol |
T24-hNectin-4 cells were resuspended in 50% Matrigel before subcutaneous injection of 5×106 T24-hNectin-4 xenograft tumor cells (0.1 mL volume) in the flanks of athymic nude female mice (Envigo, BALB/cAnNHsd). Tumor pieces were passaged via trocar needle two times to establish consistent engraftment.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Tumors were harvested 5 days after treatment and processed via polyA selection. Standard paired end read preparation by Azenta.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
1A9EVRNA2-2
|
Data processing |
Adaptors removed with cutadapt Reads were aligned and quantitated with STAR/RSEM Assembly: hg38 mm10 composite genome Supplementary files format and content: matrix of TPMs for both human and mouse reads
|
|
|
Submission date |
Apr 18, 2024 |
Last update date |
Apr 19, 2024 |
Contact name |
John Gosink |
E-mail(s) |
john@gosink.org, john.gosink@pfizer.com
|
Phone |
2062766577
|
Organization name |
Pfizer
|
Department |
Computational Biology
|
Street address |
21717 30th Dr SE
|
City |
Bothell |
State/province |
WA |
ZIP/Postal code |
98021 |
Country |
USA |
|
|
Platform ID |
GPL16512 |
Series (1) |
GSE264352 |
Enfortumab Vedotin Induces Immunogenic Cell Death and Shows Enhanced Preclinical Antitumor Activity When Combined With a PD-1 Inhibitor |
|
Relations |
BioSample |
SAMN41010296 |
SRA |
SRX24304893 |