|
| Status |
Public on Apr 19, 2024 |
| Title |
T24-hNectin4 (ADC-treated) mouse 3 |
| Sample type |
SRA |
| |
|
| Source name |
xenograft
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
tissue: xenograft
cell line: T24-hNectin4
treatment: ASG-22C3E at 3.0 mg/kg
|
| Treatment protocol |
On Day 15 post implantation, tumor volumes reached ~200 mm3 (calculated using 0.5 × [length] × [width]2), and the mice were treated with a single intraperitoneal (IP) dose of 3 mg/kg enfortumab vedotin. As a control, mice were treated with 3 mg/kg IgG-MMAE.
|
| Growth protocol |
T24-hNectin-4 cells were resuspended in 50% Matrigel before subcutaneous injection of 5×106 T24-hNectin-4 xenograft tumor cells (0.1 mL volume) in the flanks of athymic nude female mice (Envigo, BALB/cAnNHsd). Tumor pieces were passaged via trocar needle two times to establish consistent engraftment.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Tumors were harvested 5 days after treatment and processed via polyA selection.
Standard paired end read preparation by Azenta.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
1A9EVRNA2-3
|
| Data processing |
Adaptors removed with cutadapt
Reads were aligned and quantitated with STAR/RSEM
Assembly: hg38 mm10 composite genome
Supplementary files format and content: matrix of TPMs for both human and mouse reads
|
| |
|
| Submission date |
Apr 18, 2024 |
| Last update date |
Apr 19, 2024 |
| Contact name |
John Gosink |
| E-mail(s) |
john@gosink.org, john.gosink@pfizer.com
|
| Phone |
2062766577
|
| Organization name |
Pfizer
|
| Department |
Computational Biology
|
| Street address |
21717 30th Dr SE
|
| City |
Bothell |
| State/province |
WA |
| ZIP/Postal code |
98021 |
| Country |
USA |
| |
|
| Platform ID |
GPL16512 |
| Series (1) |
| GSE264352 |
Enfortumab Vedotin Induces Immunogenic Cell Death and Shows Enhanced Preclinical Antitumor Activity When Combined With a PD-1 Inhibitor |
|
| Relations |
| BioSample |
SAMN41010295 |
| SRA |
SRX24304894 |
