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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 01, 2024 |
| Title |
IS-caput, Ovch2 KO, rep1 |
| Sample type |
SRA |
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| Source name |
IS-caput epididymis
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| Organism |
Mus musculus |
| Characteristics |
genotype: Ovch2-/-
tissue: IS-caput epididymis
age: 14-week-old
treatment: no treatment
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using TRIzol (Invitrogen).
RNA libraries for RNA-seq were prepared using TruSeq stranded mRNA sample prep kit (Illumina) following manufacturer's protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
NovaSeq6000 (Illumina) using 101 bp single-ended mode
sequence reads were mapped onto mouse reference genome (mm10) using TopHat ver. 2.1.1
Fragments Per Kilobase of exon per Million mapped reads (FPKM) values for each gene were calculated using Cufflinks ver. 2.2.1
Assembly: mm10
Supplementary files format and content: A Microsoft Excel file including FPKM values for each Sample
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| Submission date |
Apr 26, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Daiji Kiyozumi |
| E-mail(s) |
kiyozumi.daiji.m6@f.mail.nagoya-u.ac.jp
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| Phone |
81-52-789-5006
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| Organization name |
Nagoya University
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| Department |
Research Institute of Environmental Medicine
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| Street address |
Furocho, Chikusa-ku
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| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8601 |
| Country |
Japan |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE265964 |
RNA sequencing analysis of Ovch2 knockout initial segment-caput epididymis. |
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| Relations |
| BioSample |
SAMN41093559 |
| SRA |
SRX24377891 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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