|
| Status |
Public on May 01, 2024 |
| Title |
Pfkfb3WT3, Kidney, Sham |
| Sample type |
SRA |
| |
|
| Source name |
kidney
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
genotype: PFKFB3 WT
treatment: Sham
|
| Treatment protocol |
The IRI mouse model was anesthetized followed by conducting clamp bilateral renal arteries for 30 min.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from kidney tissues and quantitative to 500 ng per sample was used as input material for the RNA sequence.
Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Sham_PFKFB3_WT3
|
| Data processing |
Differential expression analysis between IRI kidney with Pfkfb3ΔPTC or Pfkfb3WT was performed using the DESeq2 R package (1.16.1) and padj<=0.05 and |log2(foldchange)| >= 1 were set as the threshold for significantly differential expression.
Assembly: mm10
Supplementary files format and content: tab-delimited text file include raw counts for each sample
|
| |
|
| Submission date |
Apr 30, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
yating wang |
| E-mail(s) |
wangyt73@mail.sysu.edu.cn
|
| Phone |
15920488562
|
| Organization name |
The First Affiliated Hospital, Sun Yat-sen University
|
| Department |
Department of Nephrology
|
| Lab |
NHC Key Laboratory of Clinical Nephrology (Sun Yat-sen University) and Guangdong Provincial Key Laboratory of Nephrology
|
| Street address |
58th Zhongshan Road II The First Affiliated Hospital ,Sun Yat-Sen University
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE266242 |
PFKFB3 drives renal fibrosis through promoting histone lactylation-mediated NF-𝜅B activation [RNA-seq] |
|
| Relations |
| BioSample |
SAMN41113590 |
| SRA |
SRX24400010 |
