|
Status |
Public on Nov 11, 2011 |
Title |
Breast cancer tissue Replicate 2 |
Sample type |
RNA |
|
|
Source name |
Breast cancer tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast cancer gender: Female age: 40 years
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from fresh breast tumor and non-tumor tissues was extracted using Dr.P Kit (Biochain, Hayward, CA, USA), according to the manufacturer’s instructions.
|
Label |
Cy3
|
Label protocol |
1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
|
|
|
Hybridization protocol |
According to the instructions of Agilent Gene Expression Hybridization Kit
|
Scan protocol |
Scanned on an Agilent G2505C scanner.
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1). Agilent Feature Extraction Software (version 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Nov 03, 2011 |
Last update date |
Nov 11, 2011 |
Contact name |
wang liang |
E-mail(s) |
wangliangkexue@163.com
|
Organization name |
BioChainBJ
|
Street address |
7A North Yongchang Road BDA
|
City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
|
|
Platform ID |
GPL14550 |
Series (2) |
GSE33447 |
Gene expression profiles in breast cancer: breast cancer tissues vs. normal breast tissues |
GSE33450 |
DNA hypermethylation and gene expression in breast cancer: breast cancer tissues vs. normal breast tissues |
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