|
| Status |
Public on Nov 11, 2011 |
| Title |
Breast non-cancer tissue Replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Breast non-cancer tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal breast
gender: Female
age: NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from fresh breast tumor and non-tumor tissues was extracted using Dr.P Kit (Biochain, Hayward, CA, USA), according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
1μg of total RNA was used to synthesize the double strand cDNA. RNA was amplified by in vitro transcription using Ambion’s MessageAmp™ II aRNA Amplification Kits (Life Technologies, Austin, TX, USA). Then, aRNA was reverse transcribed into cDNA and further labeled with cy3-dCTP with Klenow enzyme.
|
| |
|
| Hybridization protocol |
According to the instructions of Agilent Gene Expression Hybridization Kit
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Agilent Feature Extraction Software (version 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Nov 03, 2011 |
| Last update date |
Nov 11, 2011 |
| Contact name |
wang liang |
| E-mail(s) |
wangliangkexue@163.com
|
| Organization name |
BioChainBJ
|
| Street address |
7A North Yongchang Road BDA
|
| City |
Beijing |
| ZIP/Postal code |
100176 |
| Country |
China |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE33447 |
Gene expression profiles in breast cancer: breast cancer tissues vs. normal breast tissues |
| GSE33450 |
DNA hypermethylation and gene expression in breast cancer: breast cancer tissues vs. normal breast tissues |
|
