|
| Status |
Public on Jun 04, 2013 |
| Title |
MelJuSo cells 1 day post etoposide + aclarubicin |
| Sample type |
RNA |
| |
|
| Source name |
MelJuSo cells 1 day post etoposide + aclarubicin
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MelJuSo
cell type: Human skin melanoma cell line
treatment: Etoposide 10uM 2 hours + Aclarubicin 10uM 2 hours
time point: 1 day post drug removal
|
| Treatment protocol |
Cells were treated with indicated drugs for 2 hours, followed by entensive washing to remove the drug.
|
| Growth protocol |
Cells were cultured in 10cm dishes and reached 80% confluency before treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction + RNeasy clean-up with DNase treatment (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Data processing |
Beadlevel data was processed using the beadarray package, affy model background corrected, log2 transformed and RSN normalized using the lumi package. All packages are available in Bioconductor.
|
| |
|
| Submission date |
Nov 11, 2011 |
| Last update date |
Jun 04, 2013 |
| Contact name |
Baoxu Pang |
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Michael Snyder
|
| Street address |
3165 Porter Drive
|
| City |
Palo Alto |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE33625 |
Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 2) |
| GSE33634 |
Topoisomerase II inhibitors and histone eviction |
|
