|
| Status |
Public on Dec 01, 2011 |
| Title |
H2O2 treated HF incubated with monocytes |
| Sample type |
RNA |
| |
|
| Source name |
HF treated with H2O2 and incubated with monocytes for 24h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin fibroblasts
treatment: H2O2
monocytes: yes
|
| Treatment protocol |
150,000 HF were treated with either PBS or with H2O2 500uM for 1h, after which they were washed and new medium was added and incubated with/without 600,000 monocytes for 24h
|
| Growth protocol |
Human fibroblats (HF) were grown in DMEM, 37C, 5% C02
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested and RNA was extracted using 5PRIME PerfectPure RNA Cultured Cell Kit (#2900319) according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000
|
| Data processing |
The data were RMA normalized
|
| |
|
| Submission date |
Nov 11, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Jacob Rachmilewitz |
| E-mail(s) |
rjacob@cc.huji.ac.il
|
| Organization name |
Hadassah hospitals
|
| Street address |
Ein Kerem
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE33631 |
Expression data from mock treated/oxidized HF after 24h incubation with/without monocytes |
|
