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GEO accession:
Sample GSM835191
Query DataSets for GSM835191
Status
Public on Jun 18, 2012
Title
2C::tomato+, WT-2
Sample type
RNA
Source name
2C::tomato+, WT-2
Organism
Mus musculus
Characteristics
cell line: ES cells
Treatment protocol
Cells were treated with vehicle or 4OHT to delete Kdm1a, and 2C::tomato+ and - cells were collected using FACS.
Growth protocol
ES cells were passaged on gelatin in ES-KOSR media
Extracted molecule
total RNA
Extraction protocol
RNA was prepared using Qiagen Rneasy kit with on-column DNAse digestion
Label
biotin
Label protocol
Labeling was perfomred with 100ng total RNA using Whole Transcript Sense target labeling kit (Affymetrix)
Hybridization protocol
25ng/ul sense DNA was hybed at 45 degrees C, washed with Affy fluidics station 4500
Scan protocol
Affymetrix 300 7G scanner
Description
Replicate 2
Data processing
RMA using Expression Console
Submission date
Nov 16, 2011
Last update date
Jun 18, 2012
Contact name
Todd Macfarlan
E-mail(s)
macfarlan@salk.edu
Organization name
The Salk Institute
Street address
10010 North Torrey Pines
City
La Jolla
State/province
CA
ZIP/Postal code
92037
Country
USA
Platform ID
GPL6246
Series (2)
GSE33763
Expression data from 2C::tomato+ vs 2C::tomato - ES cells
GSE33923
2C::tomato ES cells, 2-cell embryos and wild type oocytes
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344614
8.229779
10344616
3.166444
10344620
4.561028
10344622
8.022689
10344624
11.32631
10344633
10.87506
10344637
9.627182
10344653
5.004888
10344658
8.540354
10344674
5.068059
10344679
5.585165
10344707
9.455878
10344713
12.58965
10344715
4.6008
10344717
4.030495
10344719
6.487001
10344721
3.287176
10344723
7.611921
10344725
7.151586
10344741
11.69927
Total number of rows:
35556
Table truncated, full table size
621 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM835191.CEL.gz
4.7 Mb
(ftp)
(http)
CEL
Processed data included within Sample table
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