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Sample GSM846277 Query DataSets for GSM846277
Status Public on Dec 20, 2012
Title muscle 10-12h repliace 2
Sample type SRA
 
Source name muscle FAC sorted from dissociated embryos
Organism Drosophila melanogaster
Characteristics genetic background: P{w+mC=UAS-mCD8::GFP.L}LL6, P{w+mC=Dmef2-GAL4}
tissue: muscle
time point: 10 to 12h AEL
Growth protocol D. melanogaster embryos were collected and aged on yeasted apple juice plates at 25 °C and 60 % humidity.
Extracted molecule total RNA
Extraction protocol mRNA-seq: polyA mRNA was purified from total RNA in two consecutive rounds of oligo(dT) selection. After RNA fragmentation and reverse transcription sequencing libraries were prepared following Illumina's instructions (see ChIP-seq)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Data processing mef2_10to12h_2.bam; genome build: dm3
Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
 
Submission date Dec 08, 2011
Last update date May 15, 2019
Contact name Julia Zeitlinger
E-mail(s) jbz@stowers.org
Organization name Stowers Institute for Medical Research
Lab Zeitlinger Lab
Street address 1000 E 50th St
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
 
Platform ID GPL11203
Series (2)
GSE34281 Poised RNA Polymerase II changes over developmental time and prepares genes for future expression (RNA-seq data)
GSE34304 Poised RNA Polymerase II changes over developmental time and prepares genes for future expression
Relations
Reanalyzed by GSM3276067
SRA SRX110696
BioSample SAMN00764138

Supplementary file Size Download File type/resource
GSM846277_mef2_10to12h_2.bam 521.4 Mb (ftp)(http) BAM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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