|
| Status |
Public on Oct 10, 2012 |
| Title |
rhesus skeletal muscle |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscle
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: skeletal muscle
gender: male
library type: strand-specific, dUTP protocal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from five rhesus monkey tissues using the Trizol method and analyzed by an Agilent 2100 bio-analyzer (Agilent Technologies). Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
muscle.bam; genome build: rheMac2
muscle.bed; genome build: rheMac2
raw reads were aligned by Tophat v1.2.0, detailed parameters can be in header information of *.bam file. *.bam files and *.bed filed were used for the analysis of transcript expression and transcript structure.
|
| |
|
| Submission date |
Dec 13, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jia-Yu Chen |
| E-mail(s) |
jiayujiayu2008@163.com
|
| Organization name |
Peking University
|
| Department |
Institute of Molecular Medicine
|
| Lab |
Lab of Genomic Medicine
|
| Street address |
No.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL14954 |
| Series (1) |
| GSE34426 |
Transcriptome study in rhesus monkey |
|
| Relations |
| SRA |
SRX111327 |
| BioSample |
SAMN00765445 |
