|
Status |
Public on Nov 27, 2012 |
Title |
347520 |
Sample type |
RNA |
|
|
Source name |
ALL, total RNA
|
Organism |
Homo sapiens |
Characteristics |
pathology: ALL cytogenetics: MLL/AF4 immunophenotype: Pro-B site (pb: peripheral blood; BM: bone marrow): PB age: 59 Sex: F white blood count (wbc, 10^9/l): NA %blasts: 96% imatinib: neg overall_survival(days): 403 code_os (dead_1): 1 hyperdiploid: neg cd25(pos >=5%, neg <5%): 0
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted with Qiagen's RNeasy Plus Mini kit from Qiagen (Valencia, CA), per manufacturer's instructions.
|
Label |
Cy5/635
|
Label protocol |
Random priming with 7-mers pre-labeled with either Cy3 or Cy5 according to manufacturer's instructions using the NimbleGen Dual-Color DNA Labeling Kit (cat # 06370381001)
|
|
|
Hybridization protocol |
See Roche NimbleGen website for the detailed manufacturers protocol.
|
Scan protocol |
Scanning was performed using NimbleGen MS 200 scanner
|
Description |
14283
|
Data processing |
RMA normalization using the RMA algorithm in NimbleScan 2.5 software (Roche NimbleGen, Inc).
|
|
|
Submission date |
Jan 04, 2012 |
Last update date |
Nov 27, 2012 |
Contact name |
Huimin Geng |
E-mail(s) |
huimin.geng@ucsf.edu
|
Organization name |
UCSF
|
Department |
Department of Laboratory Medicine
|
Street address |
513 Parnassus Ave., MSB S-1480
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platform ID |
GPL15088 |
Series (2) |
GSE34861 |
Integrative Epigenomic Analysis Identifies Biomarkers and Therapeutic Targets in Adult B-Acute Lymphoblastic Leukemia [mRNA profiling] |
GSE34941 |
Integrative Epigenomic Analysis Identifies Biomarkers and Therapeutic Targets in Adult B-Acute Lymphoblastic Leukemia |
|