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Sample GSM874986 Query DataSets for GSM874986
Status Public on Feb 22, 2012
Title Fibroblast H3K27me3
Sample type SRA
 
Source name Fibroblasts differentiated from H1 ES cells (dH1f)
Organism Homo sapiens
Characteristics cell line: fibroblast derived from H1
treatment: diagnostic sample (pre-treatment)
chip antibody: H3K27me3
chip antibody manufacturer: Millipore
chip antibody catalog#: 07-449
chip antibody lot#: JBC1873477
Treatment protocol Cells were subjected to ChIP-Seq using an antibody specific for H3K27me3 or H3K79me2.
Growth protocol alpha-MEM with 10% IFS
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following Illumina ChIP-seq library preparation kit
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description Chromatin IP against H3K27me3
Data processing Sequence reads were aligned to human genome assembly hg18 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Genome Build:
Fibr.H3K27me3.n_1_l_28_e_70_a_m_1_best_strata.hg18.wig: hg18
 
Submission date Feb 13, 2012
Last update date May 15, 2019
Contact name Amit Sinha
E-mail(s) amit.sinha@childrens.harvard.edu
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
 
Platform ID GPL11154
Series (1)
GSE35791 Analysis of H3K79 methylation during reprogramming
Relations
SRA SRX119680
BioSample SAMN00788814
Named Annotation GSM874986_Fibr.H3K27me3.n_1_l_28_e_70_a_m_1_best_strata.hg18.wig.gz

Supplementary file Size Download File type/resource
GSM874986_Fibr.H3K27me3.n_1_l_28_e_70_a_m_1_best_strata.hg18.wig.gz 77.7 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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