|
| Status |
Public on Feb 22, 2012 |
| Title |
Fibroblast Epi OSKM D6 H3K79me2 |
| Sample type |
SRA |
| |
|
| Source name |
Fibroblasts differentiated from H1 ES cells (dH1f)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: fibroblast derived from H1
treatment: Cells were treated with 10uM Dot1L inhibitor (EPZ004777) for 5 days and then infected with Oct4, Sox2, Klf4 and c-Myc followed by 6 days of incubation
chip antibody: H3K79me2
chip antibody manufacturer: Abcam
chip antibody catalog#: ab3594
chip antibody lot#: 939260
|
| Treatment protocol |
Cells were subjected to ChIP-Seq using an antibody specific for H3K27me3 or H3K79me2.
|
| Growth protocol |
alpha-MEM with 10% IFS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP DNA libraries were made following Illumina ChIP-seq library preparation kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Chromatin IP against H3K79me2
|
| Data processing |
Sequence reads were aligned to human genome assembly hg18 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Genome Build:
Fibr_Epi_OSKM_D6.H3K79me2.n_1_l_28_e_70_a_m_1_best_strata.hg18.wig: hg18
|
| |
|
| Submission date |
Feb 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Amit Sinha |
| E-mail(s) |
amit.sinha@childrens.harvard.edu
|
| Phone |
617-582-7579
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Lab |
Armstrong Lab
|
| Street address |
44 Binney St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02135 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE35791 |
Analysis of H3K79 methylation during reprogramming |
|
| Relations |
| SRA |
SRX119687 |
| BioSample |
SAMN00788821 |
| Named Annotation |
GSM874993_Fibr_Epi_OSKM_D6.H3K79me2.n_1_l_28_e_70_a_m_1_best_strata.hg18.wig.gz |
