|
| Status |
Public on Jun 12, 2012 |
| Title |
Diaphragm from mdx mouse 4 |
| Sample type |
RNA |
| |
|
| Source name |
Diaphragm from mdx mouse 4
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57/B10
genotype: mdx
gender: male
age: 8 weeks
tissue: diaphragm
mouse identifier: mdx 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The heart, quadriceps femoris, and diaphragm were dissected and flash frozen in liquid nitrogen-cooled isopentane. Samples were homogenised using Precellys 24 (Bertin Technologies, France), and RNA was extracted using TRIzol reagent (Invitrogen) as according to the manufacturer’s instructions. Each RNA sample was analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA) to assess RNA quality.
|
| Label |
biotin
|
| Label protocol |
1 ug of total RNA was labeled using the FlashTag Biotin HSR kit (Genisphere).
|
| |
|
| Hybridization protocol |
Total labeled RNA was hybridised to the Affymetrix GeneChip miRNA array 2.0 using the manufacturer's protocols.
|
| Scan protocol |
Image files from scanning (.dat and .cel files) were generated with the Affymetrix Expression Console package.
|
| Description |
Dia_mdx_4
miRNA expression data from diaphragm, heart and quadriceps of mdx and WT C57/B10 mice.
|
| Data processing |
The processing and data analyses were performed in Affymetrix miRNA QCTool, as well as in DNA-Chip Analyzer (dChip) and Excel. The analysis was done according to the following workflow; BC-CG background adjustment, Quantile normalisation and Median Polish summarisation. Since the miRNA 2.0 array covers 131 organisms, we sorted out Mus musculus transcripts only.
|
| |
|
| Submission date |
Mar 04, 2012 |
| Last update date |
Jun 12, 2012 |
| Contact name |
Thomas C Roberts |
| E-mail(s) |
thomas.roberts@dpag.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Department of Physiology, Anatomy and Genetics
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE36257 |
Expression analysis in multiple muscle groups and serum reveals complexity in the microRNA transcriptome of the mdx mouse and implications for therapy |
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