|
| Status |
Public on Mar 16, 2012 |
| Title |
CSHL_RnaSeq_HUVEC_nucleus_TAP-Only_shortTotal |
| Sample type |
SRA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
biomaterial_type: primary cell
cell_type: endothelial cell of umbilical vein
donor_id: ENCDO341AAA
datatype: RnaSeq
datatype description: Sequencing analysis of RNA expression
rnaextract: shortTotal
rnaextract description: Rna shorter than 200 nt that has not been seperated based on Poly Adenalyation
biorep: 029N,030N,030N,029N
labexpid: LID21056,LID21058,LID21058,LID21056
labversion: iIDR
readtype: 1x36
readtype description: Single 36 nt reads
replicate: 3,4
localization: nucleus
localization description: Large membrane bound part of cell containing chromosomes and the bulk of the cell's DNA
protocol: TAP-Only
protocol description: This kind of library was made from RNA < 200 nucleotides that were pre-treated with Tobacco Acid Pyrophosphatase to remove any 5' caps that would preclude cloning. Hence, both capped and 5' monophosphate RNAs will be cloned.
rnaextract: shortTotal
rnaextract description: Rna shorter than 200 nt that has not been seperated based on Poly Adenalyation
biorep: 029N
labexpid: LID21056
readtype: 1x36
readtype description: Single 36 nt reads
replicate: 3
|
| Biomaterial provider |
Lonza
|
| Growth protocol |
HUVEC_protocol.pdf
|
| Extracted molecule |
nuclear RNA |
| Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeCshlShortRnaSeq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeCshlShortRnaSeq
|
| |
|
| Submission date |
Mar 16, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (1) |
| GSE24565 |
Small RNA-seq from ENCODE/Cold Spring Harbor Lab |
|
| Relations |
| SRA |
SRX130226 |
| BioSample |
SAMN00829012 |
| Named Annotation |
GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapMinusRawRep3.bigWig |
| Named Annotation |
GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapMinusRawRep4.bigWig |
| Named Annotation |
GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapPlusRawRep3.bigWig |
| Named Annotation |
GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapPlusRawRep4.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapContigs.bedRnaElements.gz |
2.3 Mb |
(ftp)(http) |
BEDRNAELEMENTS |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapExonsGencV7.gtf.gz |
2.5 Mb |
(ftp)(http) |
GTF |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapMinusRawRep3.bigWig |
14.3 Mb |
(ftp)(http) |
BIGWIG |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapMinusRawRep4.bigWig |
11.6 Mb |
(ftp)(http) |
BIGWIG |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapPlusRawRep3.bigWig |
14.3 Mb |
(ftp)(http) |
BIGWIG |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapPlusRawRep4.bigWig |
11.7 Mb |
(ftp)(http) |
BIGWIG |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapProtocolRep3.pdf.gz |
325.3 Kb |
(ftp)(http) |
PDF |
| GSM897075_hg19_wgEncodeCshlShortRnaSeqHuvecNucleusShorttotalTapProtocolRep4.pdf.gz |
325.4 Kb |
(ftp)(http) |
PDF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
