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Sample GSM897834 Query DataSets for GSM897834
Status Public on Sep 09, 2013
Title IMR90-Proliferating-4
Sample type RNA
 
Source name IMR90 fibroblasts_28 population doublings
Organism Homo sapiens
Characteristics cell line: IMR90
cell type: primary human fibroblasts
cell type: proliferating
population doublings: 28
Treatment protocol Senescence cells were maintained in dishes for 2 weeks to ensure growth termination. Senescence was determined by monitoring p16 upregulation, downregulation of cyclin genes, and by SA-beta galactosidase staining.
Growth protocol IMR90 cells (obtained from Coriell Institute for Medical Research, Camden, NJ) were grown in standard tissue culture medium (DMEM with 10% FBS and 1% penicillin streptomycin) at 3% oxygen. For senescence studies, cells were replicatively passaged until growth ceased.
Extracted molecule total RNA
Extraction protocol RNA was purified using Trizol extraction.
Label biotin
Label protocol Standard Affymetrix labeling, per manual.
 
Hybridization protocol Standard Affymetrix hybridization, per manual.
Scan protocol Standard Affymetrix scanning, per manual.
Description SAMPLE 4
Data processing Data were imported into Partek and background-subtracted using GC-RMA. Data were quantile-normalized, median-polished, and subjected to a log2 transform.
 
Submission date Mar 20, 2012
Last update date Sep 09, 2013
Contact name Gregory Donahue
Organization name The University of Pennsylvania
Department Cell & Developmental Biology
Lab Zaret Lab
Street address 3400 Civic Center Blvd, Bldg 421
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL570
Series (2)
GSE36640 Lamin B1 Depletion in Senescent Cells Triggers Large-Scale Changes in Gene Expression and in the Chromatin Landscape [ expression array ]
GSE36641 Lamin B1 depletion in senescent cells leads to large-scale changes in the chromatin landscape

Data table header descriptions
ID_REF
VALUE log2(GC-RMA signal intensity)

Data table
ID_REF VALUE
200951_s_at 1.72295
227487_s_at 8.01221
225660_at 2.22998
201310_s_at 12.7642
235753_at 7.54642
1557765_at 2.56957
231775_at 7.39242
212599_at 8.71291
229088_at 10.9796
204419_x_at 3.06718
1556834_at 7.35884
205523_at 2.44589
203180_at 9.20678
204720_s_at 2.90888
209507_at 9.4386
229005_at 3.32842
204160_s_at 2.37326
225809_at 2.3575
201477_s_at 12.7939
228361_at 6.56184

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM897834_0809_PA_3_4_nuH_IMR90_PD28_r4_2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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