|
| Status |
Public on Sep 09, 2013 |
| Title |
IMR90-Proliferating-5 |
| Sample type |
RNA |
| |
|
| Source name |
IMR90 fibroblasts_28 population doublings
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IMR90
cell type: primary human fibroblasts
cell type: proliferating
population doublings: 28
|
| Treatment protocol |
Senescence cells were maintained in dishes for 2 weeks to ensure growth termination. Senescence was determined by monitoring p16 upregulation, downregulation of cyclin genes, and by SA-beta galactosidase staining.
|
| Growth protocol |
IMR90 cells (obtained from Coriell Institute for Medical Research, Camden, NJ) were grown in standard tissue culture medium (DMEM with 10% FBS and 1% penicillin streptomycin) at 3% oxygen. For senescence studies, cells were replicatively passaged until growth ceased.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using Trizol extraction.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix labeling, per manual.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization, per manual.
|
| Scan protocol |
Standard Affymetrix scanning, per manual.
|
| Description |
SAMPLE 5
|
| Data processing |
Data were imported into Partek and background-subtracted using GC-RMA. Data were quantile-normalized, median-polished, and subjected to a log2 transform.
|
| |
|
| Submission date |
Mar 20, 2012 |
| Last update date |
Sep 09, 2013 |
| Contact name |
Gregory Donahue |
| Organization name |
The University of Pennsylvania
|
| Department |
Cell & Developmental Biology
|
| Lab |
Zaret Lab
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE36640 |
Lamin B1 Depletion in Senescent Cells Triggers Large-Scale Changes in Gene Expression and in the Chromatin Landscape [ expression array ] |
| GSE36641 |
Lamin B1 depletion in senescent cells leads to large-scale changes in the chromatin landscape |
|
