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Sample GSM898227 Query DataSets for GSM898227
Status Public on May 24, 2012
Title Input_HCT116-WT_TNF-treatment_rep2
Sample type SRA
 
Source name human colorectal carcinoma cell line HCT116
Organism Homo sapiens
Characteristics celll line: HCT116
genotype/variation: Wild type
stimulation: TNF
chip antibody: none
data processing (alignment) algorithm: ELAND
Treatment protocol Cells were treated with 10 ng/ml TNF-alpha for 30-60 mins.
Growth protocol HCT116 cells were grown in McCoys media, supplemented with 10% FBS and 1% pen-strep.
Extracted molecule genomic DNA
Extraction protocol See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
 
Data processing ChIP-Seq reads were mapped by the Eland to hg18, keeping only uniquely mapping genes and discarding all but one of clonal reads. BEDGRAPH files were generated using bed files as the input files. Only chromosome positions with at least 5 monoclonal reads are retained.
Genome_build: hg18
Supplementary_files_format_and_content: bed files, BEDGRAPH files: standard UCSC genome browser format
 
Submission date Mar 21, 2012
Last update date May 15, 2019
Contact name Bing Ren
E-mail(s) biren@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL10999
Series (1)
GSE28115 CBX3 Regulates Efficient RNA Processing Genome Wide
Relations
SRA SRX130498
BioSample SAMN00829248

Supplementary file Size Download File type/resource
GSM898227_AS18.BEDGRAPH.gz 189.4 Kb (ftp)(http) BEDGRAPH
GSM898227_AS18.bed.gz 162.6 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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