|
| Status |
Public on Jun 18, 2012 |
| Title |
G9A knockout |
| Sample type |
SRA |
| |
|
| Source name |
G9A TT2 ES cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: G9A KO
treatment: 4OHT treated
cell type: embryonic stem cells
|
| Treatment protocol |
RNA prepared by RiboPure (ambion)
|
| Growth protocol |
Stadard ES cell conditions with Lif
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TruSeq RNA sample prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Paired end alignments with Tophat allowing 4 mismatches per 100 bp
Genome Build:
G9a_KO.bed: mm9
|
| |
|
| Submission date |
Mar 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Todd Macfarlan |
| E-mail(s) |
macfarlan@salk.edu
|
| Organization name |
The Salk Institute
|
| Street address |
10010 North Torrey Pines
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE33923 |
2C::tomato ES cells, 2-cell embryos and wild type oocytes |
| GSE36896 |
RNA-Seq from wt and G9A knockout ES cells |
|
| Relations |
| SRA |
SRX132054 |
| BioSample |
SAMN00847506 |
