|
Status |
Public on Jan 30, 2014 |
Title |
Zfx fl MA9 cells + 4-OHT [10949] |
Sample type |
RNA |
|
|
Source name |
c-Kit + MA9 AML Zfx KO
|
Organism |
Mus musculus |
Characteristics |
cell line: MLL-AF9 AML cell line 10949 (Zfx fl) treatment protocol: +OHT genotype/variation: cko
|
Treatment protocol |
Cells were treated with 10nM 4-hydroxytamoxifen or ethanol vehicle
|
Growth protocol |
Cells were grown at 37 degrees and 5% carbon dioxide in RPMI + 20% FBS with glutamine, non-essential amino acids, sodium pyruvate, penicillin/streptomycin additives plus IL-3, IL-6 at 10ng/mL and SCF at 20ng/mL
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
|
|
Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
Data processing |
Gene level RMA
|
|
|
Submission date |
Mar 29, 2012 |
Last update date |
Jan 30, 2014 |
Contact name |
Stuart Weisberg |
E-mail(s) |
spw13@cumc.columbia.edu
|
Organization name |
Columbia University
|
Department |
Pathology and Cell Biology
|
Lab |
Weisberg
|
Street address |
650 W 168th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE36921 |
Effect of genetic Zfx deletion on gene expression in c-Kit + MLL-AF9 AML cells |
GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
|