|
| Status |
Public on Jan 30, 2014 |
| Title |
Zfx fl MA9 cells + 4-OHT [10950] |
| Sample type |
RNA |
| |
|
| Source name |
c-Kit + MA9 AML Zfx KO
|
| Organism |
Mus musculus |
| Characteristics |
cell line: MLL-AF9 AML cell line 10950 (Zfx fl)
treatment protocol: +OHT
genotype/variation: cko
|
| Treatment protocol |
Cells were treated with 10nM 4-hydroxytamoxifen or ethanol vehicle
|
| Growth protocol |
Cells were grown at 37 degrees and 5% carbon dioxide in RPMI + 20% FBS with glutamine, non-essential amino acids, sodium pyruvate, penicillin/streptomycin additives plus IL-3, IL-6 at 10ng/mL and SCF at 20ng/mL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
| |
|
| Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
| Data processing |
Gene level RMA
|
| |
|
| Submission date |
Mar 29, 2012 |
| Last update date |
Jan 30, 2014 |
| Contact name |
Stuart Weisberg |
| E-mail(s) |
spw13@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Pathology and Cell Biology
|
| Lab |
Weisberg
|
| Street address |
650 W 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE36921 |
Effect of genetic Zfx deletion on gene expression in c-Kit + MLL-AF9 AML cells |
| GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
|
