|
| Status |
Public on Apr 17, 2012 |
| Title |
ChIP-Seq analysis of H2A.Z in human hN r1 cells; DNA_Lib 294 |
| Sample type |
SRA |
| |
|
| Source name |
neurons derived from H9 (WA09) human stem cells; DNA_Lib 294
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: 268
sample common name: H9 Derived Neuron Cultured Cells
donor_health_status: NA
molecule: genomic DNA
disease: none
markers: >90% beta III tubulin(+) and >60% MAP2(+)
culture_conditions: Medium: Differentiated from hNP1(trademark) for 2 weeks under defined (feeder & serum free) conditions - AB2+ANS+LIF on Matrigel (i.e., differentiation medium)
passage_if_expanded: NA
cell_type: hN (ES H9 derived Neurons)
donor_ethnicity: NA
donor_sex: Female
biomaterial_type: Primary Cell Culture
donor_id: 113
biomaterial_provider: ArunA Biomedical, Athens, GA
donor_age: embryo
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H2A.Z
chip_protocol_chromatin_amount: 0.5 to 3 million cells
extraction_protocol: cell/nuclei lysis
chip_protocol_antibody_amount: ~1 ug
chip_protocol_bead_amount: 10 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: 07-594
chip_antibody_provider: Upstate
chip_antibody_lot: DAM1540736
experiment_type: Histone H2A.Z
extraction_protocol_type_of_sonicator: Branson
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sample_term_id: CL_0000540
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: DNA_Lib 294
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.9596
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.8185
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM916022_BI.H9_Derived_Neuron_Cultured_Cells.H2A.Z.DNA_Lib_294.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 294-DNA_Lib 294.hg19.level.1.release.6
ANALYSIS TITLE: Mapping of H9 Derived Neuron Cultured Cells Histone H2A.Z ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H2A.Z ChIP-Seq on H9 Derived Neuron Cultured Cells, Donor DNA_Lib 294, were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.12314
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 6
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 19,806,380
NUMBER_OF_H2A.Z_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 6
FINDPEAKS_SCORE: 0.1249
FINDPEAKS_PERCENTILE: 67
HOTSPOT_SCORE: 0.2016
HOTSPOT_PERCENTILE: 33
IROC_SCORE: 0.9797
IROC_PERCENTILE: 67
POISSON_SCORE: 0.3033
POISSON_PERCENTILE: 33
MAXIMUM_REPLICATE_CORRELATION: NA
**********************************************************************
ANALYSIS FILE NAME: GSM916022_BI.H9_Derived_Neuron_Cultured_Cells.H2A.Z.DNA_Lib_294.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 294-DNA_Lib 294.hg19.level.2.release.6
ANALYSIS TITLE: Raw Signal Density Graphs of H9 Derived Neuron Cultured Cells Histone H2A.Z ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H2A.Z ChIP-Seq read mappings from H9 Derived Neuron Cultured Cells, Donor DNA_Lib 294, were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.12361
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 6
BROWSER_TRACK_NAME: HDN H2A.Z 94
BROWSER_TRACK_DESCRIPTION: BI H9 Derived Neuron Cultured Cells Histone H2A.Z Library DNA_Lib 294 EA Release 6
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 19,806,380
NUMBER_OF_H2A.Z_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 6
FINDPEAKS_SCORE: 0.1249
FINDPEAKS_PERCENTILE: 67
HOTSPOT_SCORE: 0.2016
HOTSPOT_PERCENTILE: 33
IROC_SCORE: 0.9797
IROC_PERCENTILE: 67
POISSON_SCORE: 0.3033
POISSON_PERCENTILE: 33
MAXIMUM_REPLICATE_CORRELATION: NA
**********************************************************************
|
| |
|
| Submission date |
Apr 16, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX142754 |
| BioSample |
SAMN00691453 |
| Named Annotation |
GSM916022_BI.H9_Derived_Neuron_Cultured_Cells.H2A.Z.DNA_Lib_294.wig.gz |
