|
Status |
Public on Apr 17, 2012 |
Title |
ChIP-Seq analysis of WCE in human colonic mucosa cells; DNA_Lib 369 |
Sample type |
SRA |
|
|
Source name |
Primary colonic mucosal tissue obtained during surgery and confirmed by pathology examination to be healthy tissue; DNA_Lib 369
|
Organism |
Homo sapiens |
Characteristics |
sample alias: BioSam 157 REMC 19 sample common name: Colonic Mucosa collection_method: Surgery donor_health_status: NL colonic mucosa, colon rmvd as part of abmnl Sarc molecule: genomic DNA disease: NL colonic mucosa, colon rmvd as part of abmnl Sarc tissue_type: Colonic Mucosa donor_ethnicity: Caucasian donor_sex: Female biomaterial_type: Primary Tissue tissue_depot: Colon donor_id: 155 biomaterial_provider: MGH donor_age: 56.0 Years extraction_protocol_sonication_cycles: 1 chip_protocol_chromatin_amount: standard extraction_protocol: cell/nuclei lysis experiment_type: ChIP-Seq Input chip_protocol: Input extraction_protocol_type_of_sonicator: Branson
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Library construction protocol: Single read - Illumina
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
RANDOM |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
sample_term_id: UBERON_0000317 assay_term_id: OBI_0000716 nucleic_acid_term_id: SO_0000352 Design description: Human Chromatin IP REMC Sequencing on Illumina Library name: DNA_Lib 369 EDACC Genboree Experiment Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.9621 EDACC Genboree Sample Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.9761 **************** For data usage terms and conditions, please refer to: http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies ****************
|
Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM916047_BI.Colonic_Mucosa.Input.155.bed ANALYSIS CENTER: EDACC ANALYSIS ALIAS: DNA_Lib 369-DNA_Lib 369-DNA_Lib 369-DNA_Lib 369.hg19.level.1.release.6 ANALYSIS TITLE: Mapping of Colonic Mucosa ChIP-Seq Input Data ANALYSIS DESCRIPTION: Illumina reads produced by ChIP-Seq Input on Colonic Mucosa, Donor 155, were mapped to the human genome using Pash. ANALYSIS TYPE: REFERENCE_ALIGNMENT EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.12304 DATA_ANALYSIS_LEVEL: 1 EXPERIMENT_TYPE: ChIP-Seq GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19 SOFTWARE: Pash SOFTWARE_VERSION: 3.0 MAXIMUM_ALIGNMENT_LENGTH: Read length MISMATCHES_ALLOWED: 10% of read length ALIGNMENTS_ALLOWED: 1 TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration. TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained. ALIGNMENT_POSTPROCESSING: None READ_EXTENSION: 200bp RELEASE_NUMBER: Human Epigenome Atlas 6
QUALITY SCORES: NUMBER_OF_MAPPED_READS: 18,107,433 NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 15 FINDPEAKS_SCORE: 0.0071 FINDPEAKS_PERCENTILE: 73 HOTSPOT_SCORE: 0.0547 HOTSPOT_PERCENTILE: 73 IROC_SCORE: 0.9687 IROC_PERCENTILE: 40 POISSON_SCORE: 0.0778 POISSON_PERCENTILE: 73 MAXIMUM_REPLICATE_CORRELATION: 0.9
**********************************************************************
ANALYSIS FILE NAME: GSM916047_BI.Colonic_Mucosa.Input.155.wig ANALYSIS CENTER: EDACC ANALYSIS ALIAS: DNA_Lib 369-DNA_Lib 369-DNA_Lib 369-DNA_Lib 369.hg19.level.2.release.6 ANALYSIS TITLE: Raw Signal Density Graphs of Colonic Mucosa ChIP-Seq Input Data ANALYSIS DESCRIPTION: Illumina ChIP-Seq Input read mappings from Colonic Mucosa, Donor 155, were processed into density graphs of raw signal representing the aligned read density. ANALYSIS TYPE: ABUNDANCE_MEASUREMENT EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.12351 DATA_ANALYSIS_LEVEL: 2 EXPERIMENT_TYPE: ChIP-Seq GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19 SOFTWARE: In house programs and scripts SOFTWARE_VERSION: NA READ_EXTENSION: 200bp TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained. GENOMIC_WINDOW: 20bp TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None RELEASE_NUMBER: Human Epigenome Atlas 6 BROWSER_TRACK_NAME: CM Input 55 69 BROWSER_TRACK_DESCRIPTION: BI Colonic Mucosa ChIP-Seq Input Donor 155 Library DNA_Lib 369 EA Release 6
QUALITY SCORES: NUMBER_OF_MAPPED_READS: 18,107,433 NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 15 FINDPEAKS_SCORE: 0.0071 FINDPEAKS_PERCENTILE: 73 HOTSPOT_SCORE: 0.0547 HOTSPOT_PERCENTILE: 73 IROC_SCORE: 0.9687 IROC_PERCENTILE: 40 POISSON_SCORE: 0.0778 POISSON_PERCENTILE: 73 MAXIMUM_REPLICATE_CORRELATION: 0.9
**********************************************************************
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|
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Submission date |
Apr 16, 2012 |
Last update date |
Jan 29, 2015 |
Contact name |
BROAD INSTITUTE |
E-mail(s) |
rharris1@bcm.tmc.edu
|
Organization name |
Broad Institute
|
Street address |
-
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
Relations |
BioSample |
SAMN00857852 |
Named Annotation |
GSM916047_BI.Colonic_Mucosa.Input.155.wig.gz |