RNA extraction conducted from LeukoLock filters (Ambion, Applied Biosystems, Inc., Foster City, CA) following de-freezing. The extraction followed the manufacturers' alternative protocol instructions. Briefly, cells were flushed (TRI-ReagentTM, Ambion) into 1-bromo-3-chloropropane-containing 15ml tubes and centrifuged. 0.5 and 1.25 volume water and ethanol were added to the aqueous phase. Samples were filtered through spin cartridges, and stored in pre-heated 150 µl EDTA. RNA was quantified and examined on Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and frozen in -80⁰C.
Label
biotin
Label protocol
HJAY profiling of blood leukocytes conducted using exon array pre- prepared labeled samples using the GeneChip Whole Transcript Sense Target Labeling assay kit (Affymetrix), as per manufacturer's instructions.
Hybridization protocol
HJAY profiling of blood leukocytes conducted using exon array pre- prepared hybridization samples using the GeneChip Whole Transcript Sense Target Labeling assay kit (Affymetrix), as per manufacturer's instructions.
Scan protocol
The microarrays were scanned on an Affymetrix GeneChip Scanner 3000 7G scanner, according to the Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay protocol.
Description
J97=J69_pd12S1.CEL DBS: deep brain stimulation neurosurgery conducted to target the subthalamic nucleous (STN) brain region.
Data processing
Affymetrix Power Tools (APT version-1.10.1 ) using the RMA algorithm.
