|
| Status |
Public on Nov 20, 2013 |
| Title |
TRAIL R1 siRNA R1si_40h_3 |
| Sample type |
RNA |
| |
|
| Source name |
Panc1 cells, treated with TRAIL R1 siRNA for 40h
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: TRAIL R1 siRNA
cell line: Panc1
|
| Treatment protocol |
Panc1 cells were treated for 40h with siRNA (On-Targetplus® smart pool siRNA; Dharmacon) against TRAIL-R2 or with control siRNA in experimental setups with 5 biological replicates per group.
|
| Growth protocol |
Cells were cultured as previously described (Trauzold et al., 2001). For transient transfections DharmaFECT1 transfection reagent (Thermo Scientific, Dharmacon Epsom, UK; Panc1, MDA-MB-231, HCT116 cells) or NeoFX (Ambion, Applied Biosystems, Germany; Panc89 cells) were used. The in vitro differentiation assay of A818-6 cells was performed as described (Lehnert et al., 2001).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using miRVana (Ambion) according to the manufacturers guidelines
|
| Label |
biotin
|
| Label protocol |
according to the manufacturers guidelines (Affymetrix)
|
| |
|
| Hybridization protocol |
according to the manufacturers guidelines (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
miRNA expression profiles from cell culture
|
| Data processing |
Sample data was processed using AGCC (Affymetrix default settings) and normalized using RMA
|
| |
|
| Submission date |
May 03, 2012 |
| Last update date |
Nov 20, 2013 |
| Contact name |
Robert Haesler |
| E-mail(s) |
r.haesler@mucosa.de
|
| Phone |
+49 (0) 431 597 5754
|
| Organization name |
University Clinic of Schleswig Holstein
|
| Department |
IKMB / 1st Dept. of Medicine
|
| Lab |
Functional Genomics Platform
|
| Street address |
Schittenhelmstr. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE37734 |
miRNA expression data from human cell lines (Panc1 cells) |
|
