|
Status |
Public on May 08, 2012 |
Title |
AGS-EBV cells no vector replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
AGS-EBV cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: AGS-EBV vector: none
|
Treatment protocol |
All cells were plated at and equal density and grown for 2 days to ~50% confluence prior to harvesting for RNA prep. No G418 or puro was added to media during these two days.
|
Growth protocol |
Cells grown in F12 media with 500 ug/ml G418 to select for maintenance of EBV. Stable cells lines with pBABE vectors were generated by transfection followed by several weeks of selection with 1ug/ml puromycin until cells became resistant
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted from cell lines using Qiagen's RNeasy Plus mini kit according to manufacturer's protocol.
|
Label |
Cy5
|
Label protocol |
Agilent Quick Amp Labeling Kit was used for labeling
|
|
|
Channel 2 |
Source name |
AGS cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: AGS
|
Treatment protocol |
All cells were plated at and equal density and grown for 2 days to ~50% confluence prior to harvesting for RNA prep. No G418 or puro was added to media during these two days.
|
Growth protocol |
Cells grown in F12 media with 500 ug/ml G418 to select for maintenance of EBV. Stable cells lines with pBABE vectors were generated by transfection followed by several weeks of selection with 1ug/ml puromycin until cells became resistant
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted from cell lines using Qiagen's RNeasy Plus mini kit according to manufacturer's protocol.
|
Label |
Cy3
|
Label protocol |
Agilent Quick Amp Labeling Kit was used for labeling
|
|
|
|
Hybridization protocol |
Hybridization was performed following Agilent's Two color hybridization protocol, version 5.0.
|
Scan protocol |
Agilent DNA Microarray Scanner with Sure Scan High Resolution Technology for scanning
|
Description |
Biological Replicate 2 of 4
|
Data processing |
Raw data was processed using Aglient Feature Extraction Software. Data was analyzed using the Partek Genomics Suite 6.5
|
|
|
Submission date |
May 03, 2012 |
Last update date |
May 08, 2012 |
Contact name |
Aron Marquitz |
E-mail(s) |
arm6@med.unc.edu
|
Phone |
9199667518
|
Organization name |
University of North Carolina at Chapel Hill
|
Street address |
Lineberger Comprehensive Cancer Center
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599 |
Country |
USA |
|
|
Platform ID |
GPL14550 |
Series (1) |
GSE37753 |
AGS cells infected with Epstein Barr Virus |
|