|
| Status |
Public on May 08, 2012 |
| Title |
AGS-EBV cells pBABE replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
AGS-EBV cells with pBABE vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AGS-EBV
vector: pBABE
|
| Treatment protocol |
All cells were plated at and equal density and grown for 2 days to ~50% confluence prior to harvesting for RNA prep. No G418 or puro was added to media during these two days.
|
| Growth protocol |
Cells grown in F12 media with 500 ug/ml G418 to select for maintenance of EBV. Stable cells lines with pBABE vectors were generated by transfection followed by several weeks of selection with 1ug/ml puromycin until cells became resistant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from cell lines using Qiagen's RNeasy Plus mini kit according to manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
Agilent Quick Amp Labeling Kit was used for labeling
|
| |
|
| Channel 2 |
| Source name |
AGS cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AGS
|
| Treatment protocol |
All cells were plated at and equal density and grown for 2 days to ~50% confluence prior to harvesting for RNA prep. No G418 or puro was added to media during these two days.
|
| Growth protocol |
Cells grown in F12 media with 500 ug/ml G418 to select for maintenance of EBV. Stable cells lines with pBABE vectors were generated by transfection followed by several weeks of selection with 1ug/ml puromycin until cells became resistant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from cell lines using Qiagen's RNeasy Plus mini kit according to manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp Labeling Kit was used for labeling
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed following Agilent's Two color hybridization protocol, version 5.0.
|
| Scan protocol |
Agilent DNA Microarray Scanner with Sure Scan High Resolution Technology for scanning
|
| Description |
Biological Replicate 2 of 2
|
| Data processing |
Raw data was processed using Aglient Feature Extraction Software. Data was analyzed using the Partek Genomics Suite 6.5
|
| |
|
| Submission date |
May 03, 2012 |
| Last update date |
May 08, 2012 |
| Contact name |
Aron Marquitz |
| E-mail(s) |
arm6@med.unc.edu
|
| Phone |
9199667518
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Street address |
Lineberger Comprehensive Cancer Center
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE37753 |
AGS cells infected with Epstein Barr Virus |
|
