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| Status |
Public on Dec 11, 2012 |
| Title |
mle_2 |
| Sample type |
SRA |
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| Source name |
MLE ChIP-Seq
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| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: S2
ip antibody: monoclonal rat 6E11
shearing technologie: Covaris S220
chromatin size (bp): 180
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
As performed by sequencing service
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
service: Norwegian Sequencing Centre, Oslo, Norway
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| Data processing |
Sequenced reads were mapped to dm3 whole genome using bowtie version 0.12.7 with parameters -m 1 (single reads) and --trim3 65 -X 1500 (paired end reads)
calculation of genomic coverage vectors for each sample using a 150 bp read extension for single read samples
arcsine transformation of coverages
averaging of replicate samples
subtraction of common background (input)
Genome_build: dm3
Supplementary_files_format_and_content: bedgraph files of the mean genomic coverage
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| Submission date |
May 09, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
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| Organization name |
LMU Munich
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| Department |
Biomedical Center, Bioinformatics
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| Street address |
Großhadener Str. 9
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| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platform ID |
GPL13304 |
| Series (2) |
| GSE37864 |
ChIP-Seq profiles of MSL1, MSL2, MSl3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 in Drosophila S2 cells |
| GSE37865 |
Differential Chromatin Binding of the Drosophila Dosage Compensation Complex |
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| Relations |
| SRA |
SRX147001 |
| BioSample |
SAMN00991805 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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