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Sample GSM929771 Query DataSets for GSM929771
Status Public on Aug 16, 2012
Title MBNL2 KO, biological rep3
Sample type RNA
 
Source name wild-type hippocampus
Organism Mus musculus
Characteristics age: 2-3 mo
tissue: hippocampus
genotype: wild-type
gender: Female
genetic background: 129/BL6
Growth protocol Hippocampi dissected from MBNL2 deltaE2/deltaE2 mice or WT female mice, 2-3 months of age
Extracted molecule total RNA
Extraction protocol Mice were genotyped and whole brains were dissected from 6 mutant and 6 wild-type animals. Total RNA was extracted using Trizol.
Label biotin
Label protocol cDNA synthesis and amplification was performed using the WT Expression Kit (Ambion). Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix)
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Data processing Using Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
GPL13185_mjay.pgf
GPL13185_mjay.clf
 
Submission date May 10, 2012
Last update date Aug 16, 2012
Contact name Lily Shiue
E-mail(s) lshiue@ucsc.edu
Phone 831-459-2612
Organization name UCSC
Department MCD Biology
Lab Ares
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platform ID GPL13185
Series (1)
GSE37908 Muscleblind-Like 2 Mediated Alternative Splicing in the Developing Brain

Data table header descriptions
ID_REF
VALUE Plier normalized, background corrected summarized probeset intensities from APT.

Data table
ID_REF VALUE
127219 257.15192
58017 793.03646
28336 332.14236
564321 914.20542
544357 1402.20278
342596 1529.37249
81834 1399.41958
67525 58.58843
474416 2705.26968
598631 950.64802
403345 8791.04651
440294 245.64508
296021 6374.50437
150275 246.02646
624353 866.55005
564868 411.78417
658553 835.39908
287374 294.35585
198359 120.35652
399155 191.30081

Total number of rows: 527499

Table truncated, full table size 8635 Kbytes.




Supplementary file Size Download File type/resource
GSM929771_791_KO.CEL.gz 43.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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