|
| Status |
Public on May 01, 2013 |
| Title |
MCF-7-miR-nc |
| Sample type |
SRA |
| |
|
| Source name |
MCF-7_miR-nc
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
cell type: epithelial breast cancer cells
genotype/variation: control
|
| Treatment protocol |
MCF-7 cell lines were transiently transfected with either miR-23b or miR-26a mimic using hiperfect transfection reagent (Qiagen).
|
| Growth protocol |
Cell lines were maintained in DMEM supplemented with 10% FCS, 1% penicillin/streptomycin, and 2% glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA has been extracted using TRIzol (invitrigen). Libraries were prepared using the True-seq RNA preparation kit (Illumina) following the manufacturer instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Sequence reads were mapped to the hg19 genome assembly using Partek flow (Partek incorporated)
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using Partek Genomic Suite (Partek Incorporated)
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
May 10, 2012 |
| Last update date |
Feb 21, 2023 |
| Contact name |
Leandro Castellano |
| E-mail(s) |
l.castellano@sussex.ac.uk
|
| Organization name |
University of Sussex
|
| Street address |
Falmer, JMS Building
|
| City |
Brighton |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE37918 |
RNA-seq in breast cancer cell lines after manipulation of miR-23b expression |
|
| Relations |
| SRA |
SRX147671 |
| BioSample |
SAMN00993198 |
