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Status |
Public on Oct 18, 2012 |
Title |
PXN_C_96h |
Sample type |
RNA |
|
|
Source name |
hNT2 differentiation in presence of paraoxon
|
Organism |
Homo sapiens |
Characteristics |
cell line: hNT2 treatment: 1 µM paraoxon
|
Treatment protocol |
Paraoxon (1 µM) and mipafox (5 µM) were applied to cells freshly dissolved in the differentiation medium.
|
Growth protocol |
Undifferentiated hNT2 cells were cultured in 75 cm2 flasks at the density of 3 x 10 6 cells with 5% heat-inactivated foetal bovine serum (FBS) plus 50 U/ml penicillin and 100 µg/ml streptomycin in Opti-MEM media. After 24 hours and for initiating differentiation, a differentiation medium with 10 µM retinoic acid, 10% of FBS and antibiotics in DMEM-Glutamax was applied during 4 days. The medium was replaced at the beginning of day 3.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from hNT2 cells previously was purified using the RNeasy Plus kit (Qiagen, Germantown, MD, USA).
|
Label |
Cy3
|
Label protocol |
standard Agilent protocol
|
|
|
Hybridization protocol |
standard Agilent protocol
|
Scan protocol |
Scanned with the Agilent G2565BA Microarray Scanner (Agilent)
|
Description |
Gene expression after treatment with 1 µM paraoxon
|
Data processing |
The fluorescence intensities on scanned images were extracted and by Agilent Feature Extraction Software (v10.5.1.1). Data preprocessing was performed with the Agi4x44PreProcess R package using quantile normalization and quality filtering.
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|
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Submission date |
May 18, 2012 |
Last update date |
Oct 18, 2012 |
Contact name |
Marco Fabbri |
E-mail(s) |
fabbri.marco@gmail.com
|
Organization name |
Università dell'Insubria
|
Street address |
via dunant 5
|
City |
Varese (VA) |
ZIP/Postal code |
21100 |
Country |
Italy |
|
|
Platform ID |
GPL14550 |
Series (1) |
GSE38050 |
Genomic and phenotypic alterations of the neuronal-like cells derived from the human embryonal carcinoma stem cells (NT2) caused by exposure to the organophosphorus compounds paraoxon and mipafox |
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