|
| Status |
Public on Dec 01, 2012 |
| Title |
vehicle rep3 |
| Sample type |
RNA |
| |
|
| Source name |
vehicle rep3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human lung cancer cell line mutated in KRAS
cell line: NSCLC H358
treatment: vehicle
|
| Treatment protocol |
Cells were treated with the gamma-secretase inhibitor DAPT
|
| Growth protocol |
10% FCS in DMEM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol and then purified with Rneasy Micro kit
|
| Label |
Cy3
|
| Label protocol |
Standard
|
| |
|
| Hybridization protocol |
Standard
|
| Scan protocol |
Standard
|
| Description |
human lung cancer cell line mutated in KRAS
|
| Data processing |
Background correction was carried out using normexp, data was normalized using quantiles between-arrays normalization. Data processing was carried out using Bioconductor´s limma package.
|
| |
|
| Submission date |
May 18, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Antonio Maraver |
| E-mail(s) |
amaraver@cnio.es
|
| Organization name |
Centro Nacional de Investigaciones Oncológicas
|
| Department |
Molecular Oncology
|
| Lab |
Tumor Supression Group
|
| Street address |
Calle Melchor Fernandez Almagro 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE38054 |
Therapeutic effect of γ-secretase inhibition in KrasG12V-driven non-small cell lung carcinoma through derepression of DUSP1 phosphatase and inhibition of ERK |
|
