|
| Status |
Public on Jul 16, 2012 |
| Title |
751A3 undiff. |
| Sample type |
RNA |
| |
|
| Source name |
donor91
|
| Organism |
Homo sapiens |
| Characteristics |
disease: healthy
cell type: human iPSCs
original tissue: adult dermal fidroblast
|
| Growth protocol |
HiPSCs were maintained on feeder layers of mitomycin C-treated SNL cells in Primate ES medium (ReproCELL) supplemented with 4 ng/ml recombinant human basic fibroblast growth factor (bFGF).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with a miRNeasy mini kit (QIAGEN) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
According to manufature's protocol.
|
| |
|
| Hybridization protocol |
According to manufature's protocol.
|
| Scan protocol |
Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
|
| Description |
undifferentiated hiPSCs
|
| Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
| |
|
| Submission date |
May 23, 2012 |
| Last update date |
Jul 16, 2012 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE38153 |
aHDF- and PB-iPSC clones from the same individuals |
| GSE38155 |
Donor-dependent hepatic differentiation propensity among hiPSC lines. |
|
