|
Status |
Public on Jul 16, 2012 |
Title |
744A2 undiff. |
Sample type |
RNA |
|
|
Source name |
donor91
|
Organism |
Homo sapiens |
Characteristics |
disease: healthy cell type: human iPSCs original tissue: peripheral blood
|
Growth protocol |
HiPSCs were maintained on feeder layers of mitomycin C-treated SNL cells in Primate ES medium (ReproCELL) supplemented with 4 ng/ml recombinant human basic fibroblast growth factor (bFGF).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified with a miRNeasy mini kit (QIAGEN) according to the manufacturer’s protocol.
|
Label |
Cy3
|
Label protocol |
According to manufature's protocol.
|
|
|
Hybridization protocol |
According to manufature's protocol.
|
Scan protocol |
Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
|
Description |
undifferentiated hiPSCs
|
Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
|
|
Submission date |
May 23, 2012 |
Last update date |
Jul 16, 2012 |
Contact name |
Shinya Yamanaka |
E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
Phone |
81-75-366-7041
|
Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
|
|
Platform ID |
GPL14550 |
Series (2) |
GSE38153 |
aHDF- and PB-iPSC clones from the same individuals |
GSE38155 |
Donor-dependent hepatic differentiation propensity among hiPSC lines. |
|