|
| Status |
Public on Dec 31, 2013 |
| Title |
PC3-5_2 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 growth in BMCM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
cell type: Prostate carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
PCR product was prepared with standard Illumina protocol for arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
BeadChips were scanned using Illumina BeadArray Scanner
|
| Description |
replicate 2
|
| Data processing |
The data were normalized using quantile-normalization with GenomeStudio V2010.2
|
| |
|
| Submission date |
Jun 14, 2012 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Victor J Thannickal |
| E-mail(s) |
dip6nov@uab.edu
|
| Phone |
205-975-8938
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Medicine
|
| Lab |
Thannickal Lab
|
| Street address |
BMR2 470, 901 19th Street S
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL13376 |
| Series (1) |
| GSE38714 |
Gene expression profile in bone metastatic prostate cancer cell line, PC3, following growth in bone marrow conditioned medium (BMCM) |
|
