|
Status |
Public on Jul 16, 2012 |
Title |
control 1 |
Sample type |
RNA |
|
|
Source name |
HeLa
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa knockdown: lentiviral construct shRNA SHC002 expression: H2A.Bbd-FLAG histone
|
Treatment protocol |
Cells were transduced with a pLKO.1 lentivirus expressing a non-mammalian shRNA (SigmaAldrich SHC002V and selected with puromycin for 9 days.
|
Growth protocol |
HeLa cells were grown in DMEM with 10% calf serum
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was isolated by Trizol and sent to NimbleGen Systems Inc., Madison, WI USA for library constrcution and hybridization to the 4plex human gene expression array
|
Label |
Cy3
|
Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
|
|
Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
Description |
replicate 1
|
Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package (Roche NimbleGen, Inc.).
|
|
|
Submission date |
Jun 22, 2012 |
Last update date |
Jul 19, 2013 |
Contact name |
Peter J Park |
E-mail(s) |
peter_park@harvard.edu
|
Phone |
617-432-7373
|
Organization name |
Harvard Medical School
|
Department |
Center for Biomedical Informatics
|
Street address |
10 Shattuck St
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL8971 |
Series (2) |
GSE38771 |
Histone variant H2A.Bbd is associated with active transcription and mRNA processing in human cells |
GSE38890 |
Histone variant H2A.Bbd is associated with active transcription and mRNA processing in human cells [mRNA] |
|