|
| Status |
Public on Jul 04, 2012 |
| Title |
cervix_tumour_cs2_scc_1 |
| Sample type |
RNA |
| |
|
| Source name |
cervix tumour FFPE series 2 scc
|
| Organism |
Homo sapiens |
| Characteristics |
tissue source: clinical cervical tumour sample
tissue type: tumour
storage: formalin-fixed paraffin-embedded (FFPE)
classification: squamous cell carcinoma (SCC)
data series: 2
ffpe block storage duration time (yrs): 20
|
| Growth protocol |
Cell lines were grown in DMEM + 10% FCS. Cell lines were classified as SCC or AC according to P63 protein expression
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and Dnase treated using RecoverAll Total Nucleic Acid Isolation kit (Ambion).
|
| Label |
FlashTag-Biotin-HSR
|
| Label protocol |
Genisphere FlashTag Biotin HSR RNA labelling Kit
|
| |
|
| Hybridization protocol |
GeneChip Eukaryotic Hybridization Control Kit - Genisphere standard protocol
|
| Scan protocol |
Affymetrix GeneChip scanner 3000 running GCOS software
|
| Description |
CS2_SCC1
|
| Data processing |
Background adjustment and quantile normalisation using Affymetrix miRNA QC Tool (v.1.1.1.0)
probe group file: miRNA-2_0_v2.pgf
|
| |
|
| Submission date |
Jun 29, 2012 |
| Last update date |
Jul 04, 2012 |
| Contact name |
Jan Taylor |
| E-mail(s) |
catharine.west@manchester.ac.uk
|
| Organization name |
Paterson Institute for Cancer Research
|
| Department |
Bioinformatics
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE39015 |
Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA [Cervix samples miRNA] |
| GSE39067 |
Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA |
|
