|
| Status |
Public on Jun 30, 2012 |
| Title |
Duct7 |
| Sample type |
RNA |
| |
|
| Source name |
Duct7
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal breast duct tissue
|
| Treatment protocol |
8 breast duct samples and 8 lobule samples were obtained from premenopausal patients undergoing surgical treatment for their nipple discharge at the Guangdong Women and Children Hospital.Immediately after collection, all samples were rapidly preserved in RNAlater reagent, and a portion of each tissue sample was used for pathological examination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Trizol reagent (Invitrogen, Gaithersburg, MD) according to the supplier’s instructions.
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA each sample was amplified and labeled using Agilent LowInput Quick Amp Labeling Kit according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
According to the instructions of Agilent Gene Expression Hybridization Kit
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Description |
US10283824_252800412042
Biological replicate 7 of 8 ducts
|
| Data processing |
Agilent Feature Extraction Software (version 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 29, 2012 |
| Last update date |
Jun 30, 2012 |
| Contact name |
wang liang |
| E-mail(s) |
wangliangkexue@163.com
|
| Organization name |
BioChainBJ
|
| Street address |
7A North Yongchang Road BDA
|
| City |
Beijing |
| ZIP/Postal code |
100176 |
| Country |
China |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE39021 |
Gene expressions in normal breast duct vs lobule |
|
